ALPP and neoplasm: Immunohistochemical detection of various markers in tumor was performed by the indirect immunoperoxidase method using antibodies (AE1/AE3; Ventana, HCG; Dako, βHCG; Dako, human placental lactogen (HPL); Dako, placental alkaline phosphatase (PlAP); Dako, chromogranin-A (CGA); Dako, CD56; Leika, Insulin; Dako, Glucagon; Dako, Gastrin; Dako, Prolactin; Dako, alfa-fetoprotein(AFP); Dako, Japan) [27,28].