CD163 and infection: Therefore, to increase the infection rate of macrophage grown virus infection, 3 CHOSn-CD163 cell clones (IC5, ID9 and IF3) and 2 PK15Sn-CD163 cell clones (IXH7 and IXA3) were seeded at different densities (100 000, 200 000 or 300 000 cells/mL) and were infected at different days post seeding (1, 2 or 3 days post seeding) with 50 μL containing 104 TCID50 macrophage grown LV, after treatment of the cells with neuraminidase to remove cis-acting sialic acids.