WASF2 and infection: Infection of TZM-BL cells with A-MLV-ENV-HIV-1 or VSV-G-HIV-1 was not affected by expression of the targeted siRNAs, suggesting that Tiam-1, Abl, IRSp53, Wave2, and Arp3 are required for HIV-1 Env-mediated entry and are not necessary for post-fusion steps in the virus life cycle (Figure 3B, bottom 2 panels).