To determine if sepsis altered these activating epigenetic marks, PM were isolated from lung digest collected 24 hrs after either sham surgery or CLP, then incubated with vehicle or LPS ex vivo, and acetylation and/or trimethylation of histones binding to the murine TNF-α promoter assessed 2 hrs later by chromatin immunoprecipitation (ChIP) analysis. Here, TNF is linked to Sepsis.