In 1007 cells treatment with the PI3K inhibitor, LY294002, or with mTOR inhibitor rapamycin, or transfection with a dominant negative ILK mutant (DN-ILK) suppressed the ET-1-induced HIF-1α, HIF-2α, and VEGF expression (Figure 4B), demonstrating that ETBR-induced HIF-1α and HIF-2α accumulation and VEGF expression in melanoma cells are mediated through a PI3K-dependent ILK/AKT/mTOR signalling. Here, EDN1 is linked to melanoma.