Eight to ten days later, when tumor diameter reached 3–4 mm, mice were immunized subcutaneously four times at seven day intervals with the linear HER-GLP-1 (group 1), the branched HER-GLP-2 (group 2), both HER-GLP-1 and HER-GLP-2 (group 3) or injected with PBS alone (group 4, mock). The gene discussed is GCG; the disease is neoplasm.