SNAI2 and central nervous system cancer: To investigate whether knockdown of SNAI2/Slug could improve survival in a mouse intracranial human glioma transplantation model, we transplanted human U87 glioblastoma cells overexpressing a control vector or an shRNA vector directed against SNAI2/Slug into the brains of 5-week-old nude mice and followed the animals until they developed signs of distress due to glioblastoma growth.