Again the difference in MMP9 expression reached statistical significance only in prostate cancer samples form Gleason 8 and above (Normal vs. Gleason 6 & 7 Grade: p = 0.1517; Normal vs. Gleason 8 Grade: p = 0.0076; Normal vs. Gleason 9 & 10 Grade: p < 0.001). This evidence concerns the gene MMP9 and prostate cancer.