To this end, we first assessed the primary CD8+ T-cell response against FrCasE-infected cells during the first month post-infection by flow cytometry analysis of splenic CD8+ T lymphocytes using a MHC class I (MHC I) H2-Db tetramer loaded with the GagL peptide (Db-GagL tetramer) [32]. The gene discussed is CD8A; the disease is infection.