Consistent with the 2-DE data, the western blotting results (See additional file 4: Western blotting results of galectin-7 expression in several pairs of ESCC and NEET samples) showed elevated expression levels of galectin-7 in the ESCC samples relative to the NEET samples (Figure 4B). This evidence concerns the gene LGALS7 and esophageal squamous cell carcinoma.