SHH and neoplasm: We utilized GL26nu, GL26B6, and GL26B6V sublines to assess the influence of absent, weak, and strong anti-tumor T cell activity, respectively, on these genes, and verified up-regulation of Shh, N-myc, and CD133 in GL26B6V relative to GL26nu by quantitative PCR (qPCR; Fig. 2B and legend).