In general, the decreases in the levels of these proteins caused by metronomic gemcitabine were more pronounced than those induced by MTD gemcitabine (e.g., 21-fold vs 6-fold for CD40 ligand, 12-fold vs 4.1-fold for EGF, 34-fold vs 20-fold for GM-CSF, 57-fold vs 15-fold for ICAM-1, 397-fold vs 75-fold for IL-1α, and 189-fold vs 18-fold for IL-8, respectively, in PaCa8 tumours; 8.9-fold vs 1.9-fold for VCAM-1, 2.4-fold vs 1.7-fold for von Willebrand factor, respectively, in PaCa13 tumours). This evidence concerns the gene CD40LG and neoplasm.