Although the three melanoma cell lines represent distinct and common genetic abnormalities observed in melanoma, B16F10 (RAS), A375 (activating B-RAF, constitutively active MAPK) and MeWo (no BRAF or NRAS), they exhibited similar decreases in cell viability in the presence of increasing concentrations of thio-ccPA 18:1 (Fig. 2A). This evidence concerns the gene BRAF and melanoma.