Accordingly, percentages of CD27 IgD IgM B cells in SLE patients with negative/low levels (IIF titre ≤1/160) and medium/high levels of ANA (IIF titre ≥1/320) (Figure 2A), positive and negative anti-dsDNA Ab (Figure 2B), and positive and negative anti-ENA (extractable nuclear antigens) Ab (Figure 2C), were determined. This evidence concerns the gene CD27 and systemic lupus erythematosus.