Increased numbers and densities of LC3 punctate staining were associated with replicating H. pylori at 6 h p.i., but gradually decreased at 24 h p.i. On Western blot analysis, the signaling molecules such as LC3 II conversion, BNIP3 formation, but not in Beclin1, were also found to increase after infection compared to uninfected cells (Fig. 2B). Here, BECN1 is linked to infection.