Importantly, H-7 and staurosporine displayed a potent and dose dependent inhibition of S100A4-induced NF-κB activation in a luciferase based activity assay, both using II-11b cells (Fig. 3A and 3B) and the human osteosarcoma cell line KPDX (Fig. 3C). This evidence concerns the gene S100A4 and osteosarcoma.