IL4 and paracoccidioidomycosis: Because susceptibility to PCM is associated with the occurrence of type 2 immunity (Th2) and IL-4 was not detectable in the spleen cells supernatants, we next measured the mRNA levels of this cytokine, as well as the expression levels of GATA-3 and T-bet transcription factors, respectively associated with Th2 and Th1 cell differentiation.