In agreement with our previous observation on the high efficiency of neurogenesis from astroglia following forced Neurog2 expression, the vast majority of Neurog2-transduced astroglia had differentiated into βIII tubulin-positive, GFAP-negative neurons after 10 d in culture (Figure S1A and S1A'; 70.2%±6.3% at 9.8±3.1 days post-infection (DPI), 5 independent experiments, n = 1,022 DsRed-positive cells counted), in contrast to control retrovirus transduced cells (1.8%±1.8% of βIII tubulin-positive cells at 7.3±1.0 DPI, 3 independent experiments, n = 3,235 DsRed-positive cells counted). This evidence concerns the gene GFAP and infection.