In addition, calcium imaging experiments performed 3–4 wk post-infection in these Neurog2-reprogrammed cultures revealed a high degree of self-driven, synchronous excitatory network activity, which was blocked by CNQX and AP5 treatment (97 out of 98 DsRed-positive cells imaged; n = 3 independent experiments; Figure 13). Here, NEUROG2 is linked to infection.