Screening TLR mRNA expression at different time points after pristane injection, we found that expression of TLR1, 2, 3, 4 and 8 in the spleen is upregulated in the development stage of arthritis (d26), which is consistent with previous studies from arthritis models and RA patients [20,22,23,46]. The gene discussed is TLR1; the disease is arthritic joint disease.