In the vulvar carcinoma cell lines SW-954 and CAL-39, immunohistochemistry identified CDC25A (score = 3), CDC25B (score = 9), CDC25C (score = 3) and phospho-CDC25C (Ser216) (score = 3) in the nucleus, whereas, CDC25B (score = 3), CDC25C (SW-954, score = 6 and CAL-39, score = 9), and phospho-CDC25C (Ser216) (score = 9) were observed in the cytoplasm (Figure 3a-d). Here, CDC25B is linked to vulvar carcinoma.