In order to clarify the potential reason for sensitization of Sphk-1 inhibited macrophages to infection, we investigated the effect of SphK-1 inhibition on M. smegmatis infection induced NO generation in macrophages due to the fact that NO is a well known marker for classically activated M-1 professional macrophages and is involved in antibacterial defenses [40]–[45]. The gene discussed is SPHK1; the disease is infection.