To verify whether NK cells and/or CD8+ T cells are essential for the therapeutic effect of the MIC therapy, we performed MIC treatment in C57BL/6 wildtype mice (n = 5/group) inoculated with B16.F10 melanoma, following either a CD8+ T cell- or NK-cell depleting pre-conditioning monoclonal antibody (mAb) regimen. This evidence concerns the gene CD8A and melanoma.