CASP3 and neoplasm: As it is unclear whether direct effects of VEGF RNAi on the tumor cells synergized with DDP to induce apoptosis, we performed flow cytometry analysis, caspase-3 assay to detect apoptosis and MTT assay to measure cytotoxicity with the cultured cells transfected with the different plasmids (pshVEGF or pshHK), in presence and in absence of DDP.