In a longitudinal study of a SSc patient, it was shown by ELISA that aab reactivity to CENP-A can be induced by intra- and inter-molecular epitope spreading from histone H3 and that antibodies to CENP-A peptides can temporally precede autoreactivity to recombinant CENP-B [31]. This evidence concerns the gene CENPB and systemic sclerosis.