ISL1 and coloboma: Both of our morpholinos resulted in small eyes with a low frequency of coloboma, reduced islet-1 labeling and both were rescued by human wildtype mRNA, demonstrating the specificity of the morpholinos for TMX3. In addition, human wildtype TMX3 mRNA was able to rescue the severe reduction in islet-1 labeling exhibited in morphant eyes (Fig. 8I–J).