In this study, we demonstrate that: 1) MCF-7 breast cancer cell line expressed a classical Icilin-sensitive channel, 2) Icilin induced also an increase of intracellular Ca2+ that was mediated by endogenous plasma membrane TRPM8 activation, 3) only 30% of cells under investigation respond to Icilin, 4) expression of TRPM8 is regulated by estrogens, and 5) the over-expression of TRPM8 in breast adenocarcinomas is correlated with ER+ status. This evidence concerns the gene ESR1 and breast adenocarcinoma.