Three additional GPC-3 peptides found to have high affinity binding for HLA-A2 in the present study are thought to be less suitable for further clinical investigation because either T cells from the peripheral T cell repertoire were poorly reactive (peptide 1, GPC-3229-237 FLQALNLGI), or the epitopes were not generated by the MHC class I processing pathways in the HCC cell line used as the target cell for the CTL assay (peptide 4, GPC-3186-194 GLPDSALDI) or in the DC (peptide 5, GPC-3222-230 SLQVTRIFL). Here, GPC3 is linked to hepatocellular carcinoma.