Tumour cell and tumour microenvironmental parameters, such as the relative frequency of apoptotic cells (caspase-3 positive) within the tumours (Fig. 4A), the relative frequency of COX-2 positive cells (Fig. 4B), microvascular density (Fig. 4C), and the relative frequency of galectin-3 positive cells, used as a marker for alternatively activated M2 macrophages or dendritic cells (Fig. 4D), were analysed via immunohistochemistry of tumours obtained from the four groups studied, namely control (PBS group), DTIC or WEB2170 (DTIC or WEB groups), and combined DTIC and WEB2170 (W + D group). The gene discussed is CASP3; the disease is neoplasm.