Although it is not clear what role the ERK activation may play in the LJK-11-induced apoptosis, our data suggest that LJK-11 has different effects on cellular signaling system in comparison with other mitotic blockers and that inhibition of AKT phosphorylation/activation may be the key for LJK-11 to induce apoptosis of tumor cells. Here, AKT1 is linked to neoplasm.