Tregs within TDLN and tumors from DTA-1-treated mice showed no difference in apoptosis compared with IgG-treated controls, either by immunoflourescence (TUNEL (Figure S4A) or flow cytometric staining (Annexin V, Figure S4B, or activated caspase 3 (data not shown)) We did observe, however, that the mean fluorescence intensity (MFI) of the foxp3-GFP fusion protein was slightly lower within intra-tumor Tregs from DTA-1-treated foxp3GFP mice (Figure 5B), without changes seen in spleen and TDLN (data not shown). This evidence concerns the gene ANXA5 and neoplasm.