An important observation is that a validated set of genes (TBX5, DCC, DGKI, CDH26, HOXB13, CAVEOLIN1, PGRMC1, GNA11, TBX18, ZBTB3 and DMRT1) are indeed associated with DNMT3B and that they are methylated in more than one colon cancer cell line relative to normal colon epithelial cells (CCD841). This evidence concerns the gene PGRMC1 and malignant colon neoplasm.