TFPI free antigen levels were significantly higher in females previously suffering MI (n = 71) as compared to non-MI women (p = 0.013) (Additional file 2), mainly observed in those bearing the minor allele of the TFPI -399 and the TFPI -33 polymorphisms, and in subjects homozygous for the major TFPI -287 allele (Figure 1). This evidence concerns the gene TFPI and myocardial infarction.