NOTCH1 and cancer: Real time RT-PCR analysis with primers specific for the 5′ and 3′ regions of the Notch1 transcript and first intron showed that the higher expression of Notch1 mRNA previously reported in HKC versus keratinocyte-derived cancer cells [9], [11] was maintained irrespectively of the specific regions of the Notch1 transcript that were analyzed (Fig. 2).