6A). High expression of Pak1 protein was also correlated with shorter overall survival (P < 0.0001, Figure. 6B). Univariate analysis of overall survival with the Cox proportional hazards model revealed that tumor grade, pT stage, lymph node metastasis, LVI, active Rac1, and Pak1 were all significant variables (Table 1). This evidence concerns the gene RAC1 and metastatic malignant neoplasm in the lymph nodes.