4A). Higher expression of Pak1 protein in the primary tumor was associated with poorly differentiated cancer (grade 1; 1.67 ± 0.34, grade 2; 2.38 ± 0.96, grade 3; 3.29 ± 1.45, P < 0.0001, Figure. 4B), local invasion (<pT1; 1.89 ± 0.60, pT2; 2.52 ± 0.97, pT3; 3.09 ± 1.12, pT4; 4.56 ± 2.10, P < 0.0001, Figure. 4C), lymph node metastasis (pN0; 2.43 ± 1.09, pN1-3; 3.84 ± 1.35, P < 0.0001, Figure. 4D), and LVI (LVI(-); 2.03 ± 0.68, LVI(+); 3.34 ± 1.36, P < 0.0001, Figure. 4E). This evidence concerns the gene PAK1 and metastatic malignant neoplasm in the lymph nodes.