ATG5 and ductal breast carcinoma in situ: In DCIS progenitor cells forming spheroids or invading autologous stroma, lysosomal or autophagosome activity was up regulated in the central region of the spheroid as shown by strong fluorescence with LysoTracker Red (Figure 3F&G) and distinct staining of LC3B and Atg5 by IHC in FFPE tissue sections (Figure 3A & C).