The results showed that while the ratio between different cell populations was not significantly skewed (data not shown), the absolute cell numbers of the CD4+ T-cells, CD8+ T-cells, CD19+ B-cells and NK1.1+ NK-cells were significantly lower in the draining lymph nodes of the B16 melanoma containing RBP-J-/- DCs (Fig. 3C). This evidence concerns the gene CD8A and melanoma.