In comparison to the GL8(ABSN) and B14 reference Envs, the ABSN-B14-V5 chimera acquired the phenotype of the B14 Env, with high sensitivity to anti-CD134 (Fig. 7A, 6.2% infection at 10 μg/ml, significant increase P = 0.002) and low sensitivity to sCD134 (Fig. 7B, 65.1% infection at 10 μg/ml, significant reduction P = 0.002). Here, ERVW-1 is linked to infection.