Real-time RT-PCR shows that mRNA relative expression of E2F2 and CCND2 in prostate cancer tissues and in LNCaP, PC3, and DU-145 cells are up-regulated compared with their adjacent non-cancerous specimens and PrEC cells (Fig. 4A, B, * p<0.05; ** p<0.01). Here, CCND2 is linked to Familial prostate cancer.