Using a BRAFV600E mutant melanoma cell line with a good response to PLX4032 (M249) and another one that was poorly responsive to PLX4032 (M233) based on cell viability assays, we analyzed apoptotic induction using flow cytometry based on the incorporation of propidium iodide and Annexin V. After PLX4032-treatment, the increase in Annexin V positive cells, with or without being double positive for propidium iodide, was greater in the PLX4032-responsive M249 cells compared to the poorly responding M233 cells (Figure 2D and 2E). Here, ANXA5 is linked to melanoma.