To determine whether subcutaneous EPO+G-CSF administration could induce angiogenesis through encouraging the homing of BMSCs and their differentiation into vascular-endothelial cells at ischemic sites, double-staining immunohistochemistry, FITC-dextran perfusion studies and blood vessel density assays were performed on each brain slice from each experimental mouse at 28 days after cerebral ischemia. This evidence concerns the gene EPO and brain ischemia.