To determine whether subcutaneous EPO+G-CSF administration could induce angiogenesis through encouraging the homing of BMSCs and their differentiation into vascular-endothelial cells at ischemic sites, double-staining immunohistochemistry, FITC-dextran perfusion studies and blood vessel density assays were performed on each brain slice from each experimental mouse at 28 days after cerebral ischemia. The gene discussed is EPO; the disease is Cerebral ischemia.