EIF4G1 and nasopharyngeal carcinoma: To study the biological function of EIF4G1, we used a lentiviral vector containing shRNA specifically targeting EIF4G1 to stably knock down the endogenous expression of EIF4G1 in 5-8F cells, an NPC cell line with high levels of endogenous EIF4G1. As shown in Figure 2A, comparing to the control (shRNA-Ctrl), cells transfected with shRNA-EIF4G1 had significantly decreased levels of EIF4G1 protein.