In agreement with our previous findings [2], infection of chicken embryos with the RCABP(B)-Kir2.1 construct resulted in a significant reduction in the number of spontaneous kicks generated during a 3-minute interval in chicken embryos isolated at E8 or E10 when compared with non-infected embryos (E8 control = 12.6 ± 0.4, n = 22; E8 RCABP(B)-Kir2.1 = 1.1 ± 0.3, n = 18, P < 0.05 versus E8 control; E10 control = 25.3 ± 1.2, n = 13; E10 RCABP(B)-Kir2.1 = 5.1 ± 0.6, n = 14, P < 0.05 versus E10 control). This evidence concerns the gene KCNJ2 and infection.