IGHE and egg allergy: Here, RBL-SX38 cells or RS-ATL8 cells were similarly sensitized with serially diluted serum from egg allergy patient's (total IgE, 12 700 IU/ml; egg white specific IgE, >100 UA/ml), and stimulated with 1 μg/ml of OVA and anti-human IgE for 30 min in PIPES buffer or for 3 h in 10% FCS-containing MEM, respectively (Fig. 2).