To define mechanisms associated with the effects of MIF in P. aeruginosa keratitis we used in vitro studies to show that reduction of MIF levels by siRNA treatment or by the use of the tautomerase inhibitor of MIF, 4-IPP, results in decreased production of IL-8, IL-1β, and IL-6 by human corneal epithelial cells after infection. The gene discussed is IL6; the disease is keratitis.