Because TGF-β inhibition antagonizes the suppressive effects of MM cell conditioned media on OB differentiation (Figure 3A), we next examined whether TGF-β inhibition influences canonical Wnt signaling suppressed by MM cells, using luciferase reporter assays of the T-cell factor (TCF)/ lymphoid enhancer factor (LEF) transcription factor, a downstream target of a canonical Wnt signaling pathway. Here, HNF4A is linked to Miyoshi myopathy.