Finally, to confirm that the tumor-associated collagen detected by Masson's trichrome staining truly represented the deposition of fibrillar collagens, consecutive formalin-fixed paraffin-embedded (FFPE) glioma whole tissue sections were subject to Masson's trichrome staining and triple immunofluorescent labeling with antibodies against collagen I, collagen IV, Endo180 and GFAP (Figure 5B). The gene discussed is MRC2; the disease is neoplasm.