To further confirm that hypermethylation of the SPARC gene TRR occurs in pancreatic cancer, we also performed BSP cloning-based sequencing analysis in two pancreatic cancer cell lines (PANC1 and Patu8988), three cases of pancreatic cancer and adjacent normal tissues, two cases of normal pancreatic tissues, and two cases of WBCs from healthy volunteers. This evidence concerns the gene SPARC and familial pancreatic carcinoma.