1 and Fig. 1). Reactivity was generally cytoplasmic and membranous. The average values of WIF-1 expression were 7.33 ± 0.52 and 2.94 ± 2.19 respectively in normal brain tissues and astrocytomas. Statistical analysis indicated that the level of WIF-1 expression was significantly lower in tumors than that in normal brain tissues (P < 0.001), and it was decreased as the pathological grade increased (P = 0.002) (Tab. 2). No significant correlation was found between WIF-1 protein expression and age(P = 0.53)or sex(P = 0.69)respectively. Here, WIF1 is linked to astrocytoma (excluding glioblastoma).