For example, during growth in THY CcpA repressed the transcript level of the key virulence factor encoding genes sagA, spyCEP, and slo. However, the transcript levels of these three genes were not increased in strain 2221ΔccpA compared to strain MGAS2221 during infection Our protein-binding data indicate that binding of CcpA to cre sites in sagA, spyCEP, and slo at physiologic CcpA concentrations requires the presence of HPr-Ser46-P (Figure 3) [30],[59]. Here, KCNMA1 is linked to infection.