We show that the CR increased the expression levels of anti-inflammatory genes Il-10 and Adrbk1 (Fig. 3B and 3D) and energy homeostasis-related genes such as Ptgds (Fig. 6A), Lpin2 (Fig. 7A), Angptl6 (Fig. 7D), Kcnj11 (Fig. 6B), and Dusp9 (Fig. 8A) but were not affected by obesity in HF mice. Here, LPIN2 is linked to obesity due to melanocortin 4 receptor deficiency.